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How can I temporarily prevent access to a SharePoint site collection in SharePoint Online without removing it or deleting the user permissions?

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Lock access for SharePoint Online Site Collection.

In SharePoint Online, you can use the below PowerShell to lock access to a site collection without deleting it.

Set-SPOSite https://melqassas.sharepoint.com/sites/DemoSites -lockstate NoAccess

See also Prevent access to site collection SharePoint Server 2019

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